IMPROVED PILE CLAMP.

Pressure-polishing pipettes for improved patch-clamp recording.

Pressure-polishing is a method for shaping glass pipettes for patch-clamp recording. We first developed this method for fabricating pipettes suitable for recording from small (<3 m) neuronal cell bodies. The basic principal is similar to glass-blowing and combines air pressure and heat to modify the shape of patch pipettes prepared by a conventional micropipette puller. It can be applied to so-...

An improved vaseline gap voltage clamp for skeletal muscle fibers

A Vaseline gap potentiometric recording and voltage clamp method is developed for frog skeletal muscle fibers. The method is based on the Frankenhaeuser-Dodge voltage clamp for myelinated nerve with modifications to improve the frequency response, to compensate for external series resistance, and to compensate for the complex impedance of the current-passing pathway. Fragments of single muscle ...

Analysis of Centrifuge Pile Tests; Simulation of Pile-driving

Modeling soil pile bioremediation

A model is presented for the analysis of bioremediation applied to an ex situ soil pile. The approach is based on the remediation of a pile containing the contaminated soil, which has been excavated from its original location, placed atop a bottom liner system and encapsulated with an impermeable cover. The liner system contains and isolates the contaminated soil and provides process control po...

Pile-up glass microreactor.

We made a 'pile-up' microreactor in which ten levels of microchannel circuits were integrated to form a single glass entity. Solutions were distributed to each layer via cylindrical holes with a diameter much larger than that of the microchannel. Fabrication of the pile-up reactor was completed using only conventional photolithography, wet etching and thermal bonding techniques, and no special ...